Conjugate . Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 555 (Product # A-21422) was used at a concentration of 2 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). (H+L) Secondary Antibody, Alexa Fluor ™ 488 conjugate (Cat. The secondary antibody (yellow) was ab150078 Alexa Fluor® 555 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Antibody/Reagent Type. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Compare Secondary Antibody Search from leading suppliers on Biocompare. Secondary antibodies are raised against the host species and isotype of the primary antibody. Guaranteed product quality, expert customer support. Secondary Antibody for studying Mouse IgG in the Companion Products research area. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Anti-rat IgG (H+L) antibody was conjugated to Alexa Fluor ® 555 fluorescent dye under optimal conditions and formulated at 2 mg/mL. SLC19A2 antibody (Solute Carrier Family 19 (Thiamine Transporter), Member 2) (Alexa Fluor 555) Primary Antibody - Find MSDS or SDS, a COA, data sheets and more information. View specifications, prices, citations, reviews, and more. The GPNMB Antibody, ALEXA FLUOR® 555 Conjugated was generated using DCHIL, glycoprotein nmb, Gpnmb, HGFIN, and Osteoactivin as the antigen and it reacts with Human, Mouse, and Rat. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. Goat Secondary Antibodies Read More. Sort by. ab150078 has been referenced in 30 publications. The primary antibody concentration was 1: 100 and the secondary antibody concentration was 1:500. This data was developed using the unconjugated antibody (ab182016). The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome. The secondary antibody (yellow) was ab150078 Alexa Fluor® 555 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Please note: All products are "FOR RESEARCH USE ONLY. Unbiased reviews by scientists available at Biocompare.com. Compare the conjugates: Alexa Fluor 555 Secondary Antibody (C) vs. new Alexa Fluor Plus 555 Secondary Antibody (D). Primary antibodies; Conjugated Primary antibodies; Secondary antibodies; Conjugated Secondary antibodies ... Rabbit Anti-Chicken IgY Antibody, ALEXA FLUOR® 555 Conjugated. No. HeLa cells showing negative staining by ICC/IF using only secondary antibody. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. - Find MSDS or SDS, a COA, data sheets and more information. The secondary antibody (yellow) was ab150078 Alexa Fluor ® 555 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Alexa Fluor 555 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. Agonists, activators, antagonists and inhibitors, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (, Use at an assay dependent concentration. View Rat IgG2A Alexa Fluor® 594-conjugated Antibody (IC006T) validated in Rat. Reactivité: Humain Hôte: Souris Conjugué: Alexa Fluor 555 | Commandez IgA l'anticorps (ABIN5660092). In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Store In the Dark. citations: 12. A11008). Clonality Cat. One of my favorite secondary antibodies for red channel is Alexa Fluor 568. Store at -20°C. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Host server : magellan-srch-1-prod-blue:8080/10.253.225.14:8080. If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*. A review of the Alexa Fluor 555 Secondary Fluorescent Antibody for Immunofluorescence . Goat Secondary Antibodies. Collection Menu Refine by. Nucleus was labeled These images indicate that the Alexa Fluor ™ 488 Tyramide SuperBoost Kit is more sensitive than both the TSA ™ kits and directly labeled secondary antibodies. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Please try the standard protocols listed below and let us know how you get on. Catalog #: bs-0432R-A555. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, goat-rabbit-igg-hl-alexa-fluor-555-ab150078.pdf. Upon delivery aliquot. More than 18,000 custom antibodies created so far. CD45 Antibody, ALEXA FLUOR® 555 Conjugated Bioss Inc.'s CD45 Antibody, ALEXA FLUOR® 555 Conjugated is a Rabbit Polyclonal antibody. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact; Nominate your favourite supplier for a chance to win £100 in Amazon vouchers! If you are set on using a green channel for your primary antibody, then I would advice Alexa 488. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Actin filaments have been labeled with fluorescein phalloidin. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic … Ex: 555nm, Em: 565nm, Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. Our Abpromise guarantee covers the use of Reference; Ramaekers A, Claeys A, Kapun M, Mouchel Vielh E, Potier D, Weinberger S, et al. As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. Anti-α-Tubulin Antibody, Alexa Fluor 555 conjugate clone DM1A, from mouse, ALEXA FLUOR® 555; find Sigma-Aldrich-05-829X-555 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. We make custom antibodies for specific targets, species and applications. Alexa Fluor dyes are synthesized through sulfonation of coumarin, rhodamine, xanthene(such as fluorescein), and cyanine dyes Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Parcourir une grande sélection de produits Rabbit Secondary Antibodies et pour en savoir plus sur IgG (H+L) Rabbit anti-Mouse, Alexa Fluor® 555, Superclonal™ 1 mg; in the following tested applications. Alexa Fluor® 405/488/647/700 conjugated primary and secondary antibodies for immunofluorescence, immunohistochemistry and flow cytometry 425805 c1bbb347-4342-42ae-872c-ce0068d944dc Secondary antibody: Alexa Fluor 555-conjugated Goat Anti-Rabbit IgG (H+L) (AS058) at … To our knowledge, customised protocols are not required for this product. ... (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). Disease-modifying effects of natural ?9-tetrahydrocannabinol in endometriosis-associated pain. +41 41 420 9636 Contact; About; My Account; Log in * Hello, Guest DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. ab150078 Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Product Usage Information The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. Monoclonal Antibody for studying H3 (Lys9) acetylate in the Chromatin Regulation / Epigenetics research area. mouse and rabbit) and secondary antibodies that specifically target each species. Your browser does not have JavaScript enabled and some parts of this website will not work without it. Alexa Fluor ® 555 56 Yelow Alexa Fluor ® 594 590 617 R ed-orang Alexa Fluor ® 647 650 Red FAM 495 520 Green FITC 495 528 Green (Fluorescein Isothiocyanate) PE (Phycoerythrin) 496 and 545/566 576 Orange-yellow SureLight® P1 545 666 Red SureLight® P3 614 662 Red SureLight® PE 480 578 Yellow Spectral Red (SPRD) (PE/Cy5 ®) 565 666 Red Texas Red ® 490,675 695 Red TRITC 557 576 Orange … If you continue without changing your cookie settings, we'll assume you’re happy with this. 4E3 0.1 mg $250.00 Add to Cart. Application: Immunocytochemistry/ Immunofluorescence, Get resources and offers direct to your inbox. Narrow Search Reset All Back to Secondary Antibodies & Reagents page. Applications: IF(IHC-P) Reactivity: Chicken. Please contact us to place your order, or try again later. Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. Alexa Fluor 555 Antibody Having the excitation and emission maximum of 555/565 nm, Alexa Fluor® 555 could be effectively excited having a 543 nm He-Ne laser line and also discovered beneath normal TRITC/Cy3® filters. Use at an assay dependent dilution, Conjugation: Alexa Fluor® 555. A11008). Secondary Antibody, Alexa Fluor Plus 555 Catalog Number:A32732 Product Data Sheet Product Specific Information To minimize cross-reactivity, the goat anti-rabbit IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Peptide Synthesis Polyclonal Production Monoclonal Production Try extended incubation time. Target description The unconjugated parent antibody (Cat. Invitrogen™ Alexa Fluor 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. Images were taken and analyzed on EVOS ™ FL Auto Imaging System (Cat. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY. Alexa Fluor 555 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. Species Reactivity. Increase amount of secondary antibody used. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot. Stable for 12 months at -20°C. anti–rabbit IgG secondary antibody (as standard or highly cross-adsorbed preparations) conjugated to Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 594, or Alexa Fluor® 647, four of our most popular Alexa Fluor® dyes (Table 1). Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555. catalog : A-21435. All rights reserved. selection have been evaluated by us; we are providing them as-is and without warranty of any kind, Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Conjugate. No. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. Alexa Fluor Emission Spectra Visible Spectrum Sulfonation,What is Alexa Fluor,Alexa Fluor dyes. Read Less. Overview: Catalog # bs-23881R-A555: Product Name: CDKN2A/p16-INK4a Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated: Applications: IF(IHC-P), IF(IHC-F), IF(ICC) Price (USD) Order Qty; 9052-30 : Mouse Anti-Human IgG 1 Hinge-Alexa Fluor ® 488. For Research Use Only. Search Secondary Antibodies. Our Cookie Policy explains how you can opt-out of the cookies we use. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. scientific content. Secondary Antibody, Alexa Fluor® 555 conjugate Product Data Sheet Product Specific Information General Information The sensitivity and specificity of each lot is confirmed using ELISA. CST - Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor® 647 … No. ... > Search Results > Thermo Fisher > goat anti rabbit igg h l superclonal secondary antibody alexa fluor 555. alexa fluor 488 (Thermo Fisher ... Bioz vStars; 99. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Product Type. 35 alexa 555 Secondary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Read More. Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig and donkey IgG is observed. To test whether there was any background signal from the striatal sections of WT and Shank3 TG mice, we performed IHC experiments only with the secondary antibodies (Alexa Fluor 488 and 555). ATP Synthase (red) was labeled with anti-ATP Synthase Subunit IF1 Antibody (Cat. Invitrogen ™ Alexa Fluor Plus 555 Secondary Antibody helps you see a brighter signal over the noise. Avoid freeze / thaw cycle. Secondary antibodies are commonly conjugated with reporter molecules, either enzymes like HRP or fluorophores like APC, to enable the fluorescent, colorimetric, or chemiluminescent detection of primary antibodies. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). Comparison of the Effects of Browning-Inducing Capsaicin on Two Murine Adipocyte Models. Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact; Nominate your favourite supplier for a chance to win £100 in Amazon vouchers! If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Secondary Antibodies & Reagents. × Go to your regional site? This data was done using a primary antibody to Calmegin (EPR11832) ab171971. No. Goat Secondary Antibodies . Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. 61 reviews. ICC/IF image of ab6046 stained HeLa cells. AMCA (2) ... Alexa Fluor™ 555 (16) Alexa Fluor™ 568 (14) Alexa Fluor™ 594 (18) Alexa Fluor… This Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate is validated for use in ICC, IHC for the detection of NeuN. including for use or application of the Thermo Fisher Scientific products presented. Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. Name: Rabbit anti Goat IgG H L Superclonal Secondary Antibody. We use cookies to make our site as useful as possible. Anti-phospho-H2A.X Antibody (Ser139), clone JBW30, Alexa Fluor 555 Conjugate 1 Product Result | Match Criteria: Product Name, Description Read Less. For stable signal generation in imaging and flow cytometry, Alexa Fluor 555 dye is … B40915). Secondary (4) Secondary Antibody (962) Goat Secondary Antibodies. Antibody [A-21434] Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 by Invitrogen Antibodies. Shipped at 4°C. Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb #2317 detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Antibody (A). For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. Specificity. Overview: Catalog # bs-12148R-A555: Product Name: ZNF312 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated: Applications: IF(IHC-P), IF(IHC-F), IF(ICC) Reactivity No. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555, Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555, Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555, Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555, Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555, Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 555, Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 555. Alexa Fluor ® 405 and Alexa Fluor ® 555 and Alexa Fluor ® 647 How can I minimize cross-reactivity in multiplex experiments? No. The details of the immunogen for this antibody are not available. Store at +4°C short term (1-2 weeks). Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. © 1998-2020 Abcam plc. A21355) and then detected with Alexa Fluor ™ 594 Tyramide SuperBoost ™ Kit - Goat anti-Mouse IgG (Cat. Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078), This image is courtesy of an abreview by Zachary Yu-Ching Lin, Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078), ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078), Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078). This data is from a representative dilution. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. This antibody has been shown to work in applications such as: Immunofluorescence, Immunohistochemistry, and Immunohistochemistry - fixed. Invitrogen™ Alexa Fluor 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. The cells were incubated with the primary antibody overnight at 4°C. Figure 4. For stable signal generation in imaging and flow cytometry, Alexa Fluor 555 dye is pH-insensitive over a wide molar range. Cross-reactivity is not expected when using primary antibodies raised in different species (e.g. Insufficient antibody used Increase the amount of primary antibody used. Actin filaments have been labeled with fluorescein phalloidin. Alexa Fluor® 405/488/647/700 conjugated primary and secondary antibodies for immunofluorescence, immunohistochemistry and flow cytometry 425805 c1bbb347-4342-42ae-872c-ce0068d944dc Secondary Antibodies. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. This antibody has been shown to work in applications such as: Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunohistochemistry - fixed. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. To test whether there was any background signal from the striatal sections of WT and Shank3 TG mice, we performed IHC experiments only with the secondary antibodies (Alexa Fluor 488 and 555). Neither the content nor the BenchSci technology and processes for Donkey Anti-Mouse IgG(H+L)-Alexa Fluor 555 antibody with minimal reactivity to human, goat, sheep, bovine, rabbit, chicken, horse, and guinea pig serum proteins for use in immunocytochemistry assays. MAB377) has an observed molecular weight in the 46-48 kDa range and possibly another band at approximately 66 kDa Legal Information NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact partnerships@abcam.com, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555), Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 555). Publishing research using ab150078? Not for use in diagnostic procedures. Thermo Fisher Scientific. Please let us know so that we can cite the reference in this datasheet. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Product Description Clone Size. No. SLC19A2 antibody (Solute Carrier Family 19 (Thiamine Transporter), Member 2) (Alexa Fluor 555) Primary Antibody This Anti-Sox9 Antibody, Alexa Fluor® 555 conjugate is validated for use in Immunocytochemistry for the detection of Sox9. Secondary Antibodies Primary Antibodies Purified Ig / Isotype Controls ECM Proteins & Antibodies Assay Kits Accessory Reagents. Searching for an antibody we don't offer? AMAFD1000) using the same exposure and gain. Conjugate . (H+L) Secondary Antibody, Alexa Fluor ™ 488 conjugate (Cat. quantity : 1 mg. price : US 239. more info or order : Invitrogen product webpage. Secondary Antibodies (all) Anti-Human IgG Secondary Antibodies; Anti-Goat Secondary Antibodies; ... Chromosome 9 Open Reading Frame 142 (C9ORF142) antibody (Alexa Fluor 555) Primary Antibody. Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEET.